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Western blot analysis of extracts of various cell lines, using MYH9 antibody (13-066) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human esophagus using MYH9 antibody (13-066) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MYH9 antibody (13-066) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MYH9 antibody (13-066) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

MYH9 Antibody

CATALOG NUMBER: 13-066

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1711-1960 of human MYH9 (NP_002464.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:1000 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
LO2
Positive Control 4:
SW480
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 250kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MYH9
Additional Names:
MYH9, DFNA17, EPSTS, FTNS, MGC104539, MHA, NMHC-II-A, NMMHCA, BDPLT6, NMMHC-IIA
Ncbi Gene Id Number:
4627
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-066

List Size:
100 uL

List Price:

$436.00

Datasheet
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