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43-565 (0.3ug/ml) staining of Human peripheral blood lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-565 (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-565 (0.3ug/ml) staining of Human peripheral blood lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MYH9 Antibody

CATALOG NUMBER: 43-565

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DQINTDLNLERSH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 250kDa band observed in lysates of Human Peripheral Blood Mononucleocytes and of NIH3T3 (calculated MW of 227kDa according to Human NP_002464.1 and to Mouse NP_071855.2). Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 250kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MYH9
Additional Names:
myosin, heavy chain 9, non-muscle, RP1-68O2.1, DFNA17, EPSTS, FTNS, MGC104539, MHA, NMHC-II-A, NMMHCA, MYH9 variant protein, OTTHUMP00000028706, cellular myosin heavy chain, type A, myosin, heavy polypeptide 9, non-muscle, non-muscle myosin heavy chain, non-muscle myosin heavy polypeptide 9, nonmuscle myosin heavy chain II-A
Protein Accession Number:
NP_002464.1
Protein Gi Number:
12667788
Ncbi Gene Id Number:
4627
Background Reference 1:
Morin NA, Oakes PW, Hyun YM, Lee D, Chin YE, King MR, Springer TA, Shimaoka M, Tang JX, Reichner JS, Kim MNonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-565

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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