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Western blot analysis of extracts of various cell lines, using MYL1 antibody (23-584) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate using MYL1 antibody (23-584) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MYL1 antibody (23-584) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MYL1 antibody (23-584) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

MYL1 Antibody

CATALOG NUMBER: 23-584

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MYL1 (NP_524146.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HL-60
Positive Control 2:
Mouse heart
Positive Control 3:
Rat heart
Predicted Molecular Weight:
Observed: 27kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MYL1
Additional Names:
MLC1F, MLC3F, MYOFTA, myosin light chain 1/3, skeletal muscle isoform, A1 catalytic, A2 catalytic, MLC1/MLC3, MLC1F/MLC3F, myosin light chain A1/A2, myosin light chain alkali 1/2, myosin, light chain 1, alkali; skeletal, fast, myosin, light polypeptide 1, alkali; skeletal, fast
Ncbi Gene Id Number:
4632
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-584

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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