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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

MYLK3 Antibody

CATALOG NUMBER: 56-077

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 535-562 amino acids from the Central region of human MYLK3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYLK3
Additional Names:
Myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, MYLK3, MLCK
Protein Accession Number:
Q32MK0
Protein Gi Number:
254763411
Ncbi Gene Id Number:
91807
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphorylation of cardiac myosin heavy chains (see MYH7B,MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase,such as MYLK3, potentiates the force and rate of cross-bridgerecruitment in cardiac myocytes (Chan et al., 2008 [PubMed18202317]).
Background Reference 1:
Al-Sadi, R., et al. J. Immunol. 180(8):5653-5661(2008)
Background Reference 2:
Chan, J.Y., et al. Circ. Res. 102(5):571-580(2008)
Background Reference 3:
Seguchi, O., et al. J. Clin. Invest. 117(10):2812-2824(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-077

List Size:
400 ul

List Price:

$495.00

Datasheet
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