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Western blot analysis of extracts of various cell lines, using MYO18A antibody (23-775) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using MYO18A antibody (23-775) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

MYO18A Antibody

CATALOG NUMBER: 23-775

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1970-2054 of human MYO18A (NP_510880.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:1000 - 1:2000
Positive Control 1:
U-251MG
Positive Control 2:
HeLa
Positive Control 3:
Jurkat
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse liver
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 260kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MYO18A
Additional Names:
MAJN, MYSPDZ, SP-R21SPR21unconventional myosin-XVIIIa, SP-A receptor subunit SP-R210 alphaS, molecule associated with JAK3 N-terminus, myosin 18A, myosin containing PDZ domain, myosin containing a PDZ domain, surfactant protein receptor SP-R210
Ncbi Gene Id Number:
399687
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene can bind GOLPH3, linking the Golgi to the cytoskeleton and influencing Golgi membrane trafficking. The encoded protein is also part of a complex that assembles lamellar actomyosin bundles and may be required for cell migration.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-775

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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