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Western blot analysis of extracts of various cell lines, using MYO1C antibody (22-567) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MYO1C antibody (22-567) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MYO1C antibody (22-567).
Western blot analysis of extracts of various cell lines, using MYO1C antibody (22-567) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

MYO1C Antibody

CATALOG NUMBER: 22-567

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 769-1028 of human MYO1C (NP_203693.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
HT-29
Positive Control 2:
SKOV3
Positive Control 3:
Mouse lung
Positive Control 4:
Rat lung
Predicted Molecular Weight:
Observed: 122kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MYO1C
Additional Names:
Unconventional myosin-Ic, Myosin I beta, MMI-beta, MMIb, MYO1C
Ncbi Gene Id Number:
4641
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-567

List Size:
100 uL

List Price:

$436.00

Datasheet
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