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Western blot analysis of extracts of various cell lines, using MYO5A antibody (23-929) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using MYO5A antibody (23-929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MYO5A antibody (23-929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using MYO5A antibody (23-929) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectum using MYO5A antibody (23-929) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MYO5A antibody (23-929) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

MYO5A Antibody

CATALOG NUMBER: 23-929

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 950-1150 of human MYO5A (NP_000250.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
MCF7
Positive Control 4:
HT-1080
Positive Control 5:
Mouse spleen
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 240kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MYO5A
Additional Names:
MYO5A, MYR12, myoxin, myosin, heavy polypeptide kinase, myosin VA (heavy chain 12, myoxin), myosin VA, myosin V, MYO5, MYH12, GS1, dilute
Ncbi Gene Id Number:
4644
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-929

List Size:
100 uL

List Price:

$436.00

Datasheet
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