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42-827 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-827 (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rüdiger Rudolf, Karlsruhe, Germany
42-827 (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MYO5A Antibody

CATALOG NUMBER: 42-827

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-ETKQLELDLN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml.Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2031-6; PMID: 20133847). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MYO5A
Additional Names:
MYO5A, myosin VA (heavy chain 12, myoxin), GS1, MYH12, MYO5, MYR12, dilute, myosin V, myosin VA, myosin, heavy polypeptide kinase, myoxin
Protein Accession Number:
NP_000250.3,NP_001135967.1
Protein Gi Number:
215982791
Ncbi Gene Id Number:
4644

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-827

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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