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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MyoD (Phospho-Ser200).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MyoD (Phospho-Ser200).

MyoD (phospho Ser200) Antibody

CATALOG NUMBER: 79-443

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MyoD (Phospho-Ser200) antibody was raised against a peptide sequence around phosphorylation site of serine 200 (A-S-S (p) -P-R) derived from Human MyoD.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of MyoD only when phosphorylated at serine 200.
Predicted Molecular Weight:
40 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MYOD1
Additional Names:
PUM, MYF3, MYOD, bHLHc1, IBP3, MYOD1
Protein Accession Number:
NP_002469.2
Protein Gi Number:
23111009
Ncbi Gene Id Number:
4654
Background:
MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Background Reference 1:
Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24 (4): 1809-1821. Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19 (4): 3167-3176.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-443

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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