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Western Blot at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: RD whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with MyoD1 Antibody . Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly.
Western Blot at 1:1000 dilution Lane 1: HL-60 whole cell lysate Lane 2: RD whole cell lysate Lysates/proteins at 20 ug per lane.

MyoD1 Antibody

CATALOG NUMBER: 56-563

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYOD1
Additional Names:
Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3, MYOD1, BHLHC1, MYF3, MYOD
Protein Accession Number:
P15172
Protein Gi Number:
209572729
Ncbi Gene Id Number:
4654
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a nuclear protein that belongs to thebasic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle celldifferentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscleregeneration. It activates its own transcription which maystabilize commitment to myogenesis.
Background Reference 1:
Xynos, A., et al. Stem Cells 28(5):965-973(2010)
Background Reference 2:
Stuelsatz, P., et al. J. Biol. Chem. 285(17):12670-12683(2010)
Background Reference 3:
Hiraoka, S., et al. Hum. Pathol. 41(1):38-47(2010)
Background Reference 4:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-563

List Size:
400 ul

List Price:

$495.00

Datasheet
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