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Western blot analysis of MyoGEF Antibody in NCI-H460 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MyoGEF Antibody in NCI-H460 cell line lysates (35ug/lane)

MyoGEF Antibody

CATALOG NUMBER: 62-539

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MyoGEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-87 amino acids from the N-terminal region of human MyoGEF.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
89 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PLEKHG6
Additional Names:
Pleckstrin homology domain-containing family G member 6, PH domain-containing family G member 6, Myosin-interacting guanine nucleotide exchange factor, MyoGEF, PLEKHG6
Protein Accession Number:
Q3KR16
Protein Gi Number:
332278231
Ncbi Gene Id Number:
55200
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. It Also activates RHOG and does not activate RAC1, or to a much lower extent than RHOA and RHOG. Its part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, It is required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, it is required for normal macropinocytosis.
Background Reference 1:
D'Angelo,R., et.al., Mol. Biol. Cell 18 (12), 4780-4793 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-539

List Size:
400 ul

List Price:

$495.00

Datasheet
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