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Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin antibody (F5D)
Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin antibody (F5D)

Myogenin Antibody [F5D]

CATALOG NUMBER: 33-557

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC, IP, WB
Host Species:
Mouse
Species Reactivity:
Cat, Dog, Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Cat, Dog, Human, Mouse, Pig, Rat
Immunogen:
Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, IP, WB
Application Note:
ELISA: order BSA-free format
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Immunoprecipitation: 0.5-1 ug/500ug protein lysate
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
MYOG
Additional Names:
Myogenin, Class C basic helix-loop-helix protein 3, bHLHc3, Myogenic factor 4, Myf-4, MYOG, BHLHC3, MYF4
Ncbi Gene Id Number:
4656
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-557

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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