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43-201 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-201 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

myotilin Antibody

CATALOG NUMBER: 43-201

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DSQQHNSEHARLQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Skeletal Muscle lysates (calculated MW of 55.4kDa according to NP_006781.1. Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_006781.1; NP_001129412.1).
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
myotilin
Additional Names:
MYOT, myotilin, LGMD1, LGMD1A, TTID, 57 kDa cytoskeletal protein, OTTHUMP00000159430, OTTHUMP00000223460, OTTHUMP00000223461, myofibrillar titin-like Ig domains protein, titin immunoglobulin domain protein (myotilin)
Protein Accession Number:
NP_006781.1,NP_001129412.1
Protein Gi Number:
5803106
Ncbi Gene Id Number:
9499
Background Reference 1:
von Nandelstadh P, Soliymani R, Baumann M, Carpen O.Analysis of myotilin turnover provides mechanistic insight into the role of myotilinopathy-causing mutations.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-201

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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