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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.

MYSM1 Antibody

CATALOG NUMBER: 55-235

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MYSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the N-terminal region of human MYSM1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
95 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYSM1
Additional Names:
Histone H2A deubiquitinase MYSM1, 2A-DUB, 3419-, Myb-like, SWIRM and MPN domain-containing protein 1, MYSM1, KIAA1915
Protein Accession Number:
Q5VVJ2
Protein Gi Number:
74756898
Ncbi Gene Id Number:
114803
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation.
Background Reference 1:
Dephoure, N., et al. Proc. Natl. Acad. Sci. U.S.A. 105(31):10762-10767(2008)
Background Reference 2:
Zhu, P., et al. Mol. Cell 27(4):609-621(2007)
Background Reference 3:
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Background Reference 4:
Yoneyama, M., et al. J. Mol. Biol. 369(1):222-238(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-235

List Size:
400 ul

List Price:

$495.00

Datasheet
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