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Western blot analysis of anti-PKMYT1 Pabin A375(left) and Y79 (right)cell line lysate.
Western blot analysis of PKMYT1 using rabbit polyclonal PKMYT1 C-term using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PKMYT1 gene.
Western blot analysis of anti-PKMYT1 Pabin A375(left) and Y79 (right)cell line lysate.

MYT1 Antibody

CATALOG NUMBER: 62-719

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MYT1 (PKMYT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-482 amino acids from the C-terminal region of human MYT1 (PKMYT1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PKMYT1
Additional Names:
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, PKMYT1, MYT1
Protein Accession Number:
Q99640
Protein Gi Number:
55976573
Ncbi Gene Id Number:
9088
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Transcript Variant: This variant (1) encodes the longer isoform (1).
Background Reference 1:
Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004).
Background Reference 2:
Nakajima, H., et al., J. Biol. Chem. 278(28):25277-25280 (2003).
Background Reference 3:
Passer, B.J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2284-2289 (2003).
Background Reference 4:
Okumura, E., et al., Nat. Cell Biol. 4(2):111-116 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-719

List Size:
400 ul

List Price:

$495.00

Datasheet
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