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Western blot analysis of extracts of various cell lines, using N Cadherin antibody (18-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts from normal (control) and N Cadherin knockout (KO) HeLa cells, using N Cadherin antibody (18-917) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug N Cadherin antibody (18-917). Western blot was performed from the immunoprecipitate using N Cadherin antibody (18-917) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using N Cadherin antibody (18-917) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
KO

N Cadherin Antibody, KO Validated

CATALOG NUMBER: 18-917

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human N Cadherin (NP_001783.2).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:1000
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
A-549
Positive Control 3:
293T
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse heart
Predicted Molecular Weight:
Observed: 135kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDH2
Additional Names:
CDH2, CDw325, CDHN, Cadherin-2, CD325, CD325 antigen, N Cadherin, N-cadherin, NCAD, Neural cadherin, N-cadherin 1, Neural-cadherin
Ncbi Gene Id Number:
1000
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-917

List Size:
100 uL

List Price:

$436.00

Datasheet
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