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Western blot analysis of NRAS in A431 cell lysate with NRAS antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of NRAS in A431 cell lysate with NRAS antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

N-RAS Antibody

CATALOG NUMBER: 7381

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (93%), Chicken: (80%)
Immunogen:
N-RAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human N-RAS.
The immunogen is located within the last 50 amino acids of N-RAS.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
N-RAS antibody can be used for detection of N-RAS by Western blot at 1 and 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
N-RAS antibody is predicted to not cross-react with H-RAS
Positive Control 1:
Cat. No. 1202 – A431 Cell Lysate
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 22 kDa
Purification:
N-RAS Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
N-RAS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
N-RAS antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NRAS
Additional Names:
N-RAS Antibody: NS6, ALPS4, N-ras, NRAS1, HRAS1, GTPase NRas, Transforming protein N-Ras
Protein Accession Number:
NP_002515
Protein Gi Number:
4505451
Ncbi Gene Id Number:
4893
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Background Reference 1:
Ehrhardt A, Ehrhardt GR, Guo X, et al. Ras and relatives--job sharing and networking keep an old family together. Exp. Hematol. 2002; 30:1089-106.
Background Reference 2:
Swarthout JT, Lobo S, Farh L, et al. DHHC9 and GCP9 constitute a human protein fatty acyltransferase with specificity for H- and N-Ras. J. Biol. Chem. 2005; 280:31141-8.
Background Reference 3:
Malaney S and Daly RJ. The ras signaling pathway in mammary tumorigenesis and metastasis. J. Mammary Gland Biol. Neoplasia 2001; 6:101-13.
Background Reference 4:
Rodenhuis S. ras and human tumors. Semin Cancer Biol. 1992; 3:241-7

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7381

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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