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WB Validation in Human Cell LinesLoading: 15 μg of lysates Antibodies: N4BP1, 6175 1 μg/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 sec
Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 μg/mL.
Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 μg/mL.
WB Validation in Human Cell LinesLoading: 15 μg of lysates Antibodies: N4BP1, 6175 1 μg/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 30 sec
Trial Size

N4BP1 Antibody

CATALOG NUMBER: 6175

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.
The immunogen is located within the first 50 amino acids of N4BP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
N4BP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
N4BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
N4BP1
Additional Names:
N4BP1 Antibody: KIAA0615, NEDD4-binding protein 1, N4BP1
Protein Accession Number:
NP_694574
Protein Gi Number:
48928019
Ncbi Gene Id Number:
9683
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.
Background Reference 1:
Murillas R, Simms KS, Hatakeyama S, et al. Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. J. Biol. Chem. 2002; 277:2897-907.
Background Reference 2:
Sharma P, Murillas R, Zhang H, et al. N4BP1 is a newly identified nucleolar protein that undergoes SUMO-regulated polyubiquitylation and proteasomal turnover at promyelocytic leukemia nuclear bodies. J. Cell Sci. 2010; 123:1227-34.
Background Reference 3:
Oberst A, Malatesta M, Aqeilan RI, et al. The Nedd4-binding partner 1 (N4BP1) protein is an inhibitor of the E3 ligase Itch. Proc. Natl. Acad. Sci. USA 2007; 104:11280-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6175

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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