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Western blot analysis of extracts of various cell lines, using NAA10 antibody (22-628) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NAA10 antibody (22-628). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NAA10 antibody (22-628) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

NAA10 Antibody

CATALOG NUMBER: 22-628

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 161-235 of human NAA10 (NP_003482.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
HeLa
Positive Control 3:
HL-60
Positive Control 4:
HepG2
Positive Control 5:
HT-29
Positive Control 6:
Mouse thymus
Predicted Molecular Weight:
Observed: 26kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NAA10
Additional Names:
ARD1A, ARD1, DXS707, MGC71248, TE2, NATD, ARD1A, ARD1P, MCOPS1
Ncbi Gene Id Number:
8260
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-628

List Size:
100 uL

List Price:

$436.00

Datasheet
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