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Western blot analysis of extracts of various cell lines, using NAA60 antibody (22-906) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric using NAA60 antibody (22-906) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using NAA60 antibody (22-906) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.

NAA60 Antibody

CATALOG NUMBER: 22-906

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human NAA60 (NP_079121.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse brain
Positive Control 2:
Rat brain
Predicted Molecular Weight:
Observed: 27kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NAA60
Additional Names:
N-alpha-acetyltransferase 60, Histone acetyltransferase type B protein 4, HAT4, N-acetyltransferase 15, NatF catalytic subunit, NAA60, HAT4, NAT15
Ncbi Gene Id Number:
79903
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-906

List Size:
100 uL

List Price:

$436.00

Datasheet
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