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Western blot analysis of NAB2 antibody in A375 cell line lysates (35ug/lane)
Western blot analysis of NAB2 antibody in A375 cell line lysates (35ug/lane)

NAB2 Antibody

CATALOG NUMBER: 63-078

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NAB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human NAB2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NAB2
Additional Names:
NGFI-A-binding protein 2, EGR-1-binding protein 2, Melanoma-associated delayed early response protein, Protein MADER, NAB2, MADER
Protein Accession Number:
Q15742
Protein Gi Number:
12643729
Ncbi Gene Id Number:
4665
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NAB2 is a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by this protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex.
Background Reference 1:
Srinivasan,R., J. Biol. Chem. 281 (22), 15129-15137 (2006)
Background Reference 2:
Kumbrink,J., J. Biol. Chem. 280 (52), 42785-42793 (2005)
Background Reference 3:
Lucerna,M., J. Biol. Chem. 278 (13), 11433-11440 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-078

List Size:
400 ul

List Price:

$495.00

Datasheet
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