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NAD Synthetase M. tuberculosis Recombinant Protein

CATALOG NUMBER: 90-366

Specifications
source-species:
E. coli
source-species:
E. coli
Recombinant Protein Sequence:
NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
Fusion Tag:
His Tag
Application Note:
This recombinant proteins is for research use only.
Biological Activity:
~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1umol NAD per min.
Purity:
>98% (SDS-PAGE)
physical-state:
Liquid
Buffer:
In 20mM TRIS-HCl, pH 7.8.
Concentration:
0.5mg/ml (Lot specific)
Storage Conditions:
Stable for at least 6 months after receipt when stored at -80°C.
Additional Names:
NADE, Glutamine-dependent NAD(+) Synthetase, EC 6.3.5.1
Protein Accession Number:
P0A5L6
Background:
NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

90-366

List Size:
50 ug

List Price:

$626.00

Datasheet
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