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Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide.
Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 μg/mL.
Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide.
Trial Size

NADE Antibody

CATALOG NUMBER: 3359

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (100%)
Immunogen:
NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE.
The immunogen is located within the last 50 amino acids of NADE.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
NADE antibody can be used for detection of NADE by Western blot at 1 μg/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
NADE Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NADE Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NADE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NGFRAP1
Additional Names:
NADE Antibody: Bex, BEX3, NADE, HGR74, DXS6984E, Protein BEX3, Brain-expressed X-linked protein 3
Protein Accession Number:
NP_996798
Protein Gi Number:
46094060
Ncbi Gene Id Number:
27018
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NADE Antibody: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.
Background Reference 1:
Gentry JJ, Barker PA, and Cater BD. The p75 neurotrophin receptor: multiple interactors and numerous functions. Pro. Brain Res. 2004; 146:25-39.
Background Reference 2:
Mukai J, Hachiya T, Shoji-Hoshino S, et al. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J. Biol. Chem. 2000; 275:17566-70.
Background Reference 3:
Rapp G, Freudenstein J, Klaudiny J, et al. Characterization of three abundant mRNAs from human ovarian granulosa cells. DNA Cell Biol. 1990; 9:479-85.
Background Reference 4:
YI J-S, Lee, S-K, Sato T-A, et al. Co-induction of p75NTR and the associated death executor NADE in degenerating hippocampal neurons after kainate-induced seizures in the rat. Neurosci. Lett. 2003; 347:126-30.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3359

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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