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Antibody is used in Western blot to detect NAGK in mouse thymus tissue lysate (Lane 1) and Y79 cell lysate (Lane 2).
Antibody is used in Western blot to detect NAGK in mouse thymus tissue lysate (Lane 1) and Y79 cell lysate (Lane 2).

NAGK Antibody

CATALOG NUMBER: 62-643

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This NAGK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human NAGK.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NAGK
Additional Names:
N-acetyl-D-glucosamine kinase, N-acetylglucosamine kinase, GlcNAc kinase, NAGK
Protein Accession Number:
Q9UJ70
Protein Gi Number:
24638065
Ncbi Gene Id Number:
55577
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
N-acetylglucosamine kinase (NAGK) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals. The predicted 344-amino acid NAGK protein contains the 5 sequence motifs necessary for the binding of ATP by sugar kinases. NAGK shares 91.6% amino acid similarity with mouse Nagk, for which enzyme activity is detectable in all mouse tissues examined, with highest enzymatic activity in testis. It is hypothesized that NAGK has a general role in the catabolic pathways of GlcNAc as well as of ManNAc.
Background Reference 1:
Hinderlich, S., et al., Eur. J. Biochem. 267(11):3301-3308 (2000).
Background Reference 2:
Lowes, W., et al., Biochim. Biophys. Acta 1379(1):134-142 (1998).
Background Reference 3:
Weidanz, J.A., et al., Br. J. Haematol. 95(4):645-653 (1996).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-643

List Size:
400 ul

List Price:

$495.00

Datasheet
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