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Immunocytochemistry of NAK in MOLT4 cells with NAK antibody at 10 μg/mL.
Immunocytochemistry of NAK in MOLT4 cells with NAK antibody at 10 μg/mL.
Trial Size

NAK Antibody

CATALOG NUMBER: 2351

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
NAK antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1.
The immunogen is located within the last 50 amino acids of NAK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
NAK antibody can be used for detection of NAK/TBK1 by Western blot. An 84 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
No cross response to IKKa, IKKb, IKKy, or IKKe.
Positive Control 1:
Cat. No. 1206 - MOLT4 Cell Lysate
Positive Control 2:
Cat. No. 17-006 - MOLT-4 Cell Slide
Predicted Molecular Weight:
84 kDa
Purification:
NAK Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NAK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
NAK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TBK1
Additional Names:
NAK Antibody: NAK, T2K, NAK, NF-kappa-B-activating kinase
Protein Accession Number:
NP_037386
Protein Gi Number:
7019547
Ncbi Gene Id Number:
29110
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NAK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. Phosphorylation of I-kappaB by IκB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-κB. The IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κB-activating kinase), and T2K. NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.
Background Reference 1:
Pomerantz JL, Baltimore D. NF-κB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J 1999;18(23):6694-704
Background Reference 2:
Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S, Nakayama K, Kaneko Y, Nimura Y, Motoyama N, Ikeda K, Karin M, Nakanishi M. NAK is an IκB kinase-activating kinase. Nature 2000;404(6779):778-82
Background Reference 3:
Bonnard M, Mirtsos C, Suzuki S, Graham K, Huang J, Ng M, Itie A, Wakeham A, Shahinian A, Henzel WJ, Elia AJ, Shillinglaw W, Mak TW, Cao Z, Yeh WC. Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-κB-dependent gene transcription. EMBO J. 2000;19(18):4976-85.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2351

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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