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<strong>Figure 1 Immunofluorescence Validation of NALP1 In HeLa Cells</strong><br>Immunofluorescent analysis of methanol-fixed HeLa cells labeling NALP1 with 3037 at 20 ?g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
<strong>Figure 2 WB Validation in Human Cells</strong><br>Loading: 15 μg of lysate Antibodies: NALP1, 3037, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 3 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysate Antibodies: NALP1, 3037, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of NALP1 in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NALP1 antibody (3037) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of NALP1 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NALP1 antibody (3037) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of NALP1 in Rat Testis</strong><br> Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NALP1 antibody (3037) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of NALP1 in Human Testis</strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NALP1 antibody (3037) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Immunofluorescence Validation of NALP1 In HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling NALP1 with 3037 at 20 ?g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Trial Size

NALP1 Antibody

CATALOG NUMBER: 3037

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, ICC/IF
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1.
The immunogen is located within the last 50 amino acids of NALP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, ICC/IF
Application Note:
WB: 1 - 2 μg/mL; ICC/IF: 10-20 μg/mL; IHC-P: 1-5 μg/mL
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Predicted Molecular Weight:
Predicted: 166/157/83 kDa
Observed: 166/157/83 kDa
Purification:
NALP1 Antibody is immunoaffinity chromatography purified IgG.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NALP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NALP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NLRP1
Additional Names:
NALP1 Antibody: NAC, CARD7, CIDED, NALP1, SLEV1, DEFCAP, PP1044, VAMAS1, CLR17.1, DEFCAP-L/S, KIAA0926, NAC, NACHT, LRR and PYD domains-containing protein 1, Caspase recruitment domain-containing protein 7
Protein Accession Number:
AAG30288
Protein Gi Number:
11096303
Ncbi Gene Id Number:
22861
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NALP1 Antibody: NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.
Background Reference 1:
NALP1 antibody can be stored at 4°C, stable for one year. Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
Background Reference 2:
Chu ZL, Pio F, Xie Z, et al. A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis. J. Biol. Chem. 2001; 276:9239-45.
Background Reference 3:
Hlaing T, Guo RF, Dilley KA, et al. Molecular cloning and characterization of DEFCAP-L and -S, two isoforms of a novel member of the Ced-4 family of apoptosis proteins. J. Biol. Chem. 2001; 276:9230-8.
Background Reference 4:
Sanz C, Calasanz MJ, Andreu E, et al. NALP1 is a transcriptional target for cAMP-response-element-binding protein (CREB) in myeloid leukemia cells. Biochem. J. 2004; 384:281-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3037

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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