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<strong>Figure 1 Immunofluorescence Validation of NALP1 In HeLa Cells</strong><br>Immunofluorescent analysis of methanol-fixed HeLa cells labeling NALP1 with 3037 at 20 ?g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
<strong>Figure 2 WB Validation in Human Cells</strong><br>Loading: 15 μg of lysate Antibodies: NALP1, 3037, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 3 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysate Antibodies: NALP1, 3037, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of NALP1 in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NALP1 antibody (3037) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of NALP1 in Mouse Brain</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NALP1 antibody (3037) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of NALP1 in Rat Testis</strong><br> Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NALP1 antibody (3037) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of NALP1 in Human Testis</strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-NALP1 antibody (3037) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Immunofluorescence Validation of NALP1 In HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling NALP1 with 3037 at 20 ?g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Green Product
Trial Size

NALP1 Antibody

CATALOG NUMBER: 3037

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, ICC/IF
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1.
The immunogen is located within the last 50 amino acids of NALP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, ICC/IF
Application Note:
WB: 1 - 2 μg/mL; ICC/IF: 10-20 μg/mL; IHC-P: 1-5 μg/mL
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Predicted Molecular Weight:
Predicted: 166/157/83 kDa
Observed: 166/157/83 kDa
Purification:
NALP1 Antibody is immunoaffinity chromatography purified IgG.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
NALP1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NALP1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NLRP1
Additional Names:
NALP1 Antibody: NAC, CARD7, CIDED, NALP1, SLEV1, DEFCAP, PP1044, VAMAS1, CLR17.1, DEFCAP-L/S, KIAA0926, NAC, NACHT, LRR and PYD domains-containing protein 1, Caspase recruitment domain-containing protein 7
Protein Accession Number:
AAG30288
Protein Gi Number:
11096303
Ncbi Gene Id Number:
22861
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NALP1 Antibody: NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.
Background Reference 1:
NALP1 antibody can be stored at 4°C, stable for one year. Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
Background Reference 2:
Chu ZL, Pio F, Xie Z, et al. A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis. J. Biol. Chem. 2001; 276:9239-45.
Background Reference 3:
Hlaing T, Guo RF, Dilley KA, et al. Molecular cloning and characterization of DEFCAP-L and -S, two isoforms of a novel member of the Ced-4 family of apoptosis proteins. J. Biol. Chem. 2001; 276:9230-8.
Background Reference 4:
Sanz C, Calasanz MJ, Andreu E, et al. NALP1 is a transcriptional target for cAMP-response-element-binding protein (CREB) in myeloid leukemia cells. Biochem. J. 2004; 384:281-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3037

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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