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Western blot analysis of NALP4 in K562 cell lysate with NALP4 antibody at 1 μg/mL.
Western blot analysis of NALP4 in K562 cell lysate with NALP4 antibody at 1 μg/mL.
Trial Size

NALP4 Antibody

CATALOG NUMBER: 5957

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
NALP4 antibody was raised against a 13 amino acid synthetic peptide near the center of human NALP4.
The immunogen is located within amino acids 330 - 380 of NALP4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NALP4 antibody can be used for detection of NALP4 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
NALP4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NALP4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NALP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NLRP4
Additional Names:
NALP4 Antibody: CT58, PAN2, RNH2, NALP4, PYPAF4, CLR19.5, NACHT, LRR and PYD domains-containing protein 4, PAAD and NACHT-containing protein 2, CT58
Protein Accession Number:
AAL35293
Protein Gi Number:
17064172
Ncbi Gene Id Number:
147945
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NALP4 Antibody: NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP4, also known as PYRIN-containing APAF1-like protein 4, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. In transfected 293 cells, NALP4 suppressed NF-κB induction by TNF-α and IL-1β, suggesting NALP4 operates at a point of convergence in these two signaling pathways. NALP4 also suppressed NF-κB induction resulting from overexpression of several adapter proteins and protein kinases involved in these pathways, including TRAF2, TRAF6, RIP, and IRAK2, as well as the IKK-alpha and IKK-beta, suggesting that NALP4 is critical in modulating NF-κB activity.
Background Reference 1:
Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104.
Background Reference 2:
Tian X, Pascal G, and Monget P. Evolution and functional divergence of NLRP genes in mammalian reproductive system. BMC Evol. Biol.2009; 9:202.
Background Reference 3:
Fiortino L, Stehlik C, Oliveira V, et al. A novel PAAD-containing protein that modulates NF-kappa B induction by cytokines tumor necrosis factor-alpha and interleukin-1beta. J. Biol. Chem.2002; 277:35333-40.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5957

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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