Nampt Antibody

CATALOG NUMBER: 36-226

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide corresponding to aa 413-431 (V413FKDPVADPNKRSKKGRLS431) of human Nampt (Visfatin/PBEF).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections, 10ug/ml). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Specificity:
Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Storage Conditions:
Stable for at least 6 months after receipt when stored at -20°C.
Ncbi Official Symbol:
NAMPT
Additional Names:
Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Protein Accession Number:
5031977
Protein Gi Number:
NP_005737
Ncbi Gene Id Number:
10135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

36-226

List Size:
100 ug

List Price:

$463.00

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