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Western blot analysis of lysate from MCF-7 cell line, using NAMPT Antibody at 1:1000.
Western blot analysis of NAMPT Antibody in A375 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with NAMPT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from MCF-7 cell line, using NAMPT Antibody at 1:1000.

NAMPT Antibody

CATALOG NUMBER: 63-743

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This NAMPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-426 amino acids from the Central region of human NAMPT.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NAMPT
Additional Names:
Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor, Visfatin, NAMPT, PBEF, PBEF1
Protein Accession Number:
P43490
Protein Gi Number:
1172027
Ncbi Gene Id Number:
10135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NAMPT catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Background Reference 1:
Samal,B., et.al., Mol. Cell. Biol. 14 (2), 1431-1437 (1994)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-743

List Size:
400 ul

List Price:

$495.00

Datasheet
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