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Nampt Antibody [OMNI379]

CATALOG NUMBER: 36-034

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, ICC, IHC, IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human Nampt (visfatin/PBEF).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, IHC, IP, WB
Application Note:
ELISA: (direct or indirect: 1:2,000-1:10,000). Flow Cytometry: (1:1,000). Immunocytochemistry: (1:500-1:1,000). Immunohistochemistry: (paraffin sections, 1:100-1:500). Immunoprecipitation: (1:500-1:1,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Specificity:
Recognizes human, mouse and rat Nampt.
Clonality:
Monoclonal
Isotype:
IgG2a, kappa
physical-state:
Liquid
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Storage Conditions:
Stable for at least 1 year after receipt when stored at -20°C.
Ncbi Official Symbol:
NAMPT
Additional Names:
Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Protein Accession Number:
5031977
Protein Gi Number:
NP_005737
Ncbi Gene Id Number:
10135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

36-034

List Size:
100 ug

List Price:

$515.00

Datasheet
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