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45-932 (0.05ug/ml) staining of HeLa (nuclear) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-932 (5ug/ml) as the reporter antibody.
45-932 (2ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-932 (0.05ug/ml) staining of HeLa (nuclear) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NANOG Antibody

CATALOG NUMBER: 45-932

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DLFLNYSMNMQPED
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1ug/ml.An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB06860.
Positive Control 1:
Cat. No. XBL-10433 - HeLa Nuclear Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
NANOG
Additional Names:
NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Protein Accession Number:
NP_079141.2
Protein Gi Number:
13376298
Ncbi Gene Id Number:
79923
Background Reference 1:
Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H. NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer. Genes Cells. 2006 Sep;11(9):1115-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-932

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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