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Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using Nano g Antibody.
Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using Nano g Antibody.

Nanog Antibody

CATALOG NUMBER: 79-644

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Nanog antibody was raised against a peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:1000
Specificity:
This antibody detects endogenous level of total Nanog protein.
Predicted Molecular Weight:
42 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
NANOG
Additional Names:
FLK1, VEGFR2, Homeobox transcription factor Nanog
Protein Accession Number:
NP_079141.2
Protein Gi Number:
153945816
Ncbi Gene Id Number:
79923
Background:
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation
Background Reference 1:
Do HJ, et al. Biochem Biophys Res Commun. 2007 Feb 16;353 (3): 770-5. Boyer LA, et al. Cell. 2005 Sep 23;122 (6): 947-56. Freberg CT, et al. Mol Biol Cell. 2007 May;18 (5): 1543-53. Chambers I, et al. Cell. 2003 May 30;113 (5): 643-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-644

List Size:
0.1 mg

List Price:

$370.00

Datasheet
Shipping Info

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