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Western blot analysis of lysates from NCCIT cells using Nanog Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from F9 cells, using 5 μg of Nanog Rabbit mAb and Rabbit Control IgG. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
Flow cytometry: 1X10^6 HeLa cells (negative control,left) and NTERA-2 cells (right) were intracellularly-stained with Nanog Rabbit mAb or Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 NTERA-2 cells were intracellularly-stained with Rabbit IgG isotype control or Nanog Rabbit mAb, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.
Flow cytometry: 1X10^6 NIH/3T3 cells (negative control,left) and F9 cells (right) were intracellularly-stained with Nanog Rabbit mAb or Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 F9 cells were intracellularly-stained with Rabbit IgG isotype control or Nanog Rabbit mAb, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.
Western blot analysis of lysates from NCCIT cells using Nanog Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

Nanog Rabbit Recombinant Antibody (83-179)

CATALOG NUMBER: 83-179

Clonality:
Monoclonal
Tested Applications:
ChIP, ELISA, FC (intra), WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, FC (intra), WB
Application Note:
WB,1:500 - 1:1000, ChIP,5μg antibody for 10μg-15μg of Chromatin, FC (intra),1:500 - 1:1000
Predicted Molecular Weight:
34kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Nanog
Additional Names:
ENK; Stm1; ecat4; 2410002E02Rik
Protein Accession Number:
Q80Z64
Ncbi Gene Id Number:
71950
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-179

List Size:
100 uL

List Price:

$477.00

Datasheet
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