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Western blot analysis of Nanos2 in human testis lyate with Nanos2 antibody at (A) 2 and (B) 4 μg/mL.
Western blot analysis of Nanos2 in human testis lyate with Nanos2 antibody at (A) 2 and (B) 4 μg/mL.
Trial Size

Nanos2 Antibody

CATALOG NUMBER: 4645

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Nanos2 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of the human Nanos2.
The immunogen is located within the last 50 amino acids of Nanos2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Nanos2 antibody can be used for detection of Nanos2 by Western blot at 2 to 4 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
This Nanos2 antibody will not cross-react with either Nanos 1 or Nanos3.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Nanos2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Nanos2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Nanos2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NANOS2
Additional Names:
Nanos2 Antibody: NOS2, NOS2, Nanos homolog 2, NOS-2
Protein Accession Number:
P60321
Protein Gi Number:
41688561
Ncbi Gene Id Number:
339345
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nanos2 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.
Background Reference 1:
Lehmann R and Nusslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the drosophila embryo. Development1991; 112:679-91.
Background Reference 2:
Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science2003; 301:1239-41.
Background Reference 3:
Sato K, Hayashi Y, Ninomiya Y, et al. Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. Proc. Natl. Acad. Sci. USA2007; 104:7455-60.
Background Reference 4:
Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev.2003; 120:721-31.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4645

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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