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Western blot analysis of Nanos3 in human brain tissue lysate with Nanos3 antibody at 2 μg/mL.
Immunohistochemistry of Nanos3 in human brain tissue with Nanos3 antibody at 2.5 μg/mL.
Immunofluorescence of Nanos3 in Human Brain tissue with Nanos3 antibody at 20 μg/mL.
Western blot analysis of Nanos3 in human brain tissue lysate with Nanos3 antibody at 2 μg/mL.
Trial Size

Nanos3 Antibody

CATALOG NUMBER: 4653

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Nanos3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3.
The immunogen is located within the last 50 amino acids of Nanos3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Nanos3 antibody can be used for detection of Nanos3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 19 kDa
Observed: 20 kDa
Purification:
Nanos3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Nanos3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Nanos3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NANOS3
Additional Names:
Nanos3 Antibody: NOS3, NANOS1L, NOS3, Nanos homolog 3, NOS-3
Protein Accession Number:
P60323
Protein Gi Number:
41688563
Ncbi Gene Id Number:
342977
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Nanos3 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.
Background Reference 1:
Lehmann R and Nusslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the drosophila embryo. Development 1991; 112:679-91.
Background Reference 2:
Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science 2003; 301:1239-41.
Background Reference 3:
Sato K, Hayashi Y, Ninomiya Y, et al. Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. Proc. Natl. Acad. Sci. USA 2007; 104:7455-60.
Background Reference 4:
Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev. 2003; 120:721-31.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4653

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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