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To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human NAP-2 (XP-5244) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNAP-2.
To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human NAP-2 (XP-5244) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNAP-2.

NAP-2 Antibody (biotin)

CATALOG NUMBER: XP-5244Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNAP-2 (human NAP-2).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hNAP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human NAP-2 (XP-5244) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2.
Western Blot:
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hNAP-2 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
NAP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
PPBP
Additional Names:
PBP, TC1, TC2, TGB, LDGF, MDGF, TGB1, B-TG1, CTAP3, CXCL7, NAP-2, SCYB7, THBGB, LA-PF4, THBGB1, Beta-TG, CTAPIII, CTAP-III, Platelet basic protein, C-X-C motif chemokine 7, PBP
Protein Accession Number:
P02775
Protein Gi Number:
129874
Ncbi Gene Id Number:
5473
User Note:
Centrifuge vial prior to opening.
Background:
NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5244Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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