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Western blot analysis of extracts of various cell lines, using NAP1L2 antibody (14-254) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using NAP1L2 antibody (14-254) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

NAP1L2 Antibody

CATALOG NUMBER: 14-254

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NAP1L2 (NP_068798.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
U-87MG
Positive Control 2:
Jurkat
Positive Control 3:
Mouse kidney
Positive Control 4:
Rat brain
Positive Control 5:
Rat kidney
Predicted Molecular Weight:
Observed: 55kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NAP1L2
Additional Names:
Nucleosome assembly protein 1-like 2, Brain-specific protein, X-linked, NAP1L2, BPX
Ncbi Gene Id Number:
4674
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this intronless gene is a member of the nucleosome assembly protein (NAP) family. The encoded protein represents a class of tissue-specific factors that interact with chromatin to regulate neuronal cell proliferation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-254

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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