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Western blot analysis of NAP1L5 in EL4 cell lysate with NAP1L5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 5 μg/mL.
Immunofluorescence of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 20 μg/mL.
Western blot analysis of NAP1L5 in EL4 cell lysate with NAP1L5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

NAP1L5 Antibody

CATALOG NUMBER: 8147

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
NAP1L5 antibody was raised against an 18 amino acid peptide near the center of human NAP1L5.
The immunogen is located within amino acids 40 - 90 of NAP1L5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NAP1L5 antibody can be used for detection of NAP1L5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
NAP1L5 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Predicted Molecular Weight:
Predicted: 20 kDa (17kDa in mouse)
Observed: 15 kDa
Purification:
NAP1L5 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NAP1L5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NAP1L5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NAP1L5
Additional Names:
Nucleosome assembly protein 1-like 5, DRLM, down-regulated in liver malignancy
Protein Accession Number:
NP_715638
Protein Gi Number:
24371268
Ncbi Gene Id Number:
266812
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The nucleosome assembly protein 1-like 5 (NAP1L5) protein, also known as DRLM, was initially identified as a possible tumor suppressor gene in hepatocellular carcinoma (1). The NAP1L5 gene is located within the intron of Herc3, and unlike Herc3, is imprinted and highly expressed in regions of the adult mouse brain (2).
Background Reference 1:
Harada H, Nagai H, Ezura Y, et al. Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein. Gene 2002; 296:171-7.
Background Reference 2:
Davies W, Smith RJ, Kelsey G, et al. Expression patterns of the novel imprinted genes Nap1L5 and Peg13 and their non-imprinted host genes in the adult mouse brain. Gene Expr. Patterns 2004; 4:741-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8147

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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