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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
NAPSA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

NAPSA Antibody

CATALOG NUMBER: 57-260

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NAPSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-397 amino acids from the C-terminal region of human NAPSA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NAPSA
Additional Names:
Napsin-A, 3423-, Aspartyl protease 4, ASP4, Asp 4, Napsin-1, TA01/TA02, NAPSA, NAP1, NAPA
Protein Accession Number:
O96009
Ncbi Gene Id Number:
9476
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The activation peptides of aspartic proteinases plays roleas inhibitors of the active site. These peptide segments, orpro-parts, are deemed important for correct folding, targeting, andcontrol of the activation of aspartic proteinase zymogens. Thepronapsin A gene is expressed predominantly in lung and kidney. Itstranslation product is predicted to be a fully functional,glycosylated aspartic proteinase precursor containing an RGD motifand an additional 18 residues at its C-terminus. [provided byRefSeq].
Background Reference 1:
Bishop, J.A., et al. Hum. Pathol. 41(1):20-25(2010)
Background Reference 2:
Woischnik, M., et al. Eur. Respir. J. 31(6):1197-1204(2008)
Background Reference 3:
Ueno, T., et al. Lab. Invest. 88(3):256-263(2008)
Background Reference 4:
Innocenti, M., et al. Nat. Cell Biol. 6(4):319-327(2004)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-260

List Size:
400 ul

List Price:

$495.00

Datasheet
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