Western blot analysis of NARS using rabbit polyclonal NARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.

NARS Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in WiDr,HepG2 cell line lysates (35ug/lane).

Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of NARS using rabbit polyclonal NARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.

NARS Antibody

CATALOG NUMBER: 55-618

Clonality:

Polyclonal

Tested Applications:

Flow, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Homology:

Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse

Immunogen:

This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human NARS.

Conjugate:

Unconjugated

Tested Applications:

Flow, IHC-P, WB

Application Note:

For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

63 kDa

Advanced Validation

Properties

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

NARS

Additional Names:

Asparagine--tRNA ligase, cytoplasmic, Asparaginyl-tRNA synthetase, AsnRS, NARS

Protein Accession Number:

O43776

Ncbi Gene Id Number:

4677

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

Aminoacyl-tRNA synthetases are a class of enzymes thatcharge tRNAs with their cognate amino acids. Asparaginyl-tRNAsynthetase is localized to the cytoplasm and belongs to the classII family of tRNA synthetases. The N-terminal domain representsthe signature sequence for the eukaryotic asparaginyl-tRNAsynthetases.

Background Reference 1:

Lim, J., et al. Cell 125(4):801-814(2006)

Background Reference 2:

Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)

Background Reference 3:

Shiba, K., et al. Nucleic Acids Res. 26(22):5045-5051(1998)

Background Reference 4:

Beaulande, M., et al. Nucleic Acids Res. 26(2):521-524(1998)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-618

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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NARS Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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