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Western blot analysis of NAT11 in human thymus tissue lysate with NAT11 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of NAT11 in mouse thymus tissue with NAT11 antibody at 5 μg/mL.
Immunofluorescence of NAT11 in mouse thymus tissue with NAT11 antibody at 20 μg/mL.
Western blot analysis of NAT11 in human thymus tissue lysate with NAT11 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NAT11 Antibody

CATALOG NUMBER: 5549

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
NAT11 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human NAT11.
The immunogen is located within the last 50 amino acids of NAT11.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NAT11 antibody can be used for detection of NAT11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Purification:
NAT11 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NAT11 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NAT11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NAA40
Additional Names:
NAT11 Antibody: NAT11, PATT1, NAT11, N-alpha-acetyltransferase 40, N-acetyltransferase 11
Protein Accession Number:
NP_079047
Protein Gi Number:
189571650
Ncbi Gene Id Number:
79829
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NAT11 Antibody: N-terminal acetylation is one of the most common protein modifications in eukaryotes, occurring on approximately 57% and 84% on yeast and human proteins respectively. There are several N-terminal acetylating enzyme complexes (NatA - NatE). Unlike the other complexes, NatD is composed of a single protein, NAT11, and has recently been described to acetylate the Serine N-termini of histones H2A and H4 in yeast. The role these modifications play is unknown; yeast that do not express NAT11 grow at normal rates and have no observable phenotypes. The role of the human homolog is likewise unknown.
Background Reference 1:
Arnesen T, Van Damme P, Polevoda B, et al. Proteomics analyses reveal the evolutionary conservation and divergence of N-terminal acetyltransferases from yeast and humans. Proc. Natl. Acad. Sci. USA2009; 106:8157-62.
Background Reference 2:
OK Song, Wang X, Waterborg JH, et al. An Nalpha-acetyl-transferase responsible for acetylation of the N-terminal residues of histones H4 and H2A. J. Biol. Chem.2003; 278:38109-1

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5549

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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