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Western blot analysis of extracts of various cell lines, using NAV2 antibody (16-535) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung using NAV2 antibody (16-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using NAV2 antibody (16-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using NAV2 antibody (16-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NAV2 antibody (16-535) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using NAV2 antibody (16-535) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using NAV2 antibody (16-535) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

NAV2 Antibody

CATALOG NUMBER: 16-535

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1800-1980 of human NAV2 (NP_660093.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
HepG2
Positive Control 2:
MCF-7
Positive Control 3:
A-431
Predicted Molecular Weight:
Observed: 100kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NAV2
Additional Names:
NAV2, neuron navigator 2, HELAD1, POMFIL2, RAINB1, STEERIN2, UNC53H2, helicase, APC down-regulated 1, pore membrane and/or filament-interacting-like protein 2, retinoic acid inducible gene in neuroblastoma 1, steerin-2, unc-53 homolog 2
Ncbi Gene Id Number:
89797
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the neuron navigator gene family, which may play a role in cellular growth and migration. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-535

List Size:
100 uL

List Price:

$436.00

Datasheet
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