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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).

NCALD Antibody

CATALOG NUMBER: 55-219

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig, Rat, Chicken, Monkey, Xenopus
Immunogen:
This NCALD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-174 amino acids from the C-terminal region of human NCALD.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCALD
Additional Names:
Neurocalcin-delta, NCALD
Protein Accession Number:
P61601
Protein Gi Number:
47606440
Ncbi Gene Id Number:
83988
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NCALD encodes a member of the neuronal calcium sensor(NCS) family of calcium-binding proteins. The protein contains anN-terminal myristoylation signal and four EF-hand calcium bindingloops. The protein is cytosolic at resting calcium levels; however,elevated intracellular calcium levels induce a conformationalchange that exposes the myristoyl group, resulting in proteinassociation with membranes and partial co-localization with theperinuclear trans-golgi network. The protein is thought to be aregulator of G protein-coupled receptor signal transduction.Several alternatively spliced variants of this gene have beendetermined, all of which encode the same protein; additionalvariants may exist but their biological validity has not beendetermined.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)
Background Reference 3:
Gill, M.B., et al. J. Biol. Chem. 284(21):14503-14512(2009)
Background Reference 4:
Venkataraman, V., et al. Biochemistry 47(25):6590-6601(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-219

List Size:
400 ul

List Price:

$495.00

Datasheet
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