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Western blot analysis of extracts of mouse brain, using NCAM1 / CD56 antibody (13-162).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of mouse brain, using NCAM1 / CD56 antibody (13-162).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

NCAM1 / CD56 Antibody

CATALOG NUMBER: 13-162

Clonality:
Polyclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human NCAM1 / CD56
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:100
Positive Control 1:
Mouse brain tissue
Predicted Molecular Weight:
Observed: 120kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NCAM1
Additional Names:
neural cell adhesion molecule, NCAM, antigen recognized by monoclonal 5.1H11, NCAM-1, N-CAM-1, MSK39, CD56, neural cell adhesion molecule 1, NCAM1
Ncbi Gene Id Number:
4684
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-162

List Size:
100 uL

List Price:

$436.00

Datasheet
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