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Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

NCAPG2 Antibody

CATALOG NUMBER: 58-920

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NCAPG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 729-757 amino acids from the Central region of human NCAPG2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
131 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCAPG2
Additional Names:
Condensin-2 complex subunit G2, Chromosome-associated protein G2, CAP-G2, hCAP-G2, Leucine zipper protein 5, Non-SMC condensin II complex subunit G2, NCAPG2, LUZP5
Protein Accession Number:
Q86XI2
Protein Gi Number:
74727834
Ncbi Gene Id Number:
54892
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Condensin complexes I and II play essential roles inmitotic chromosome assembly and segregation. Both condensinscontain 2 invariant structural maintenance of chromosome (SMC)subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they containdifferent sets of non-SMC subunits. NCAPG2 is 1 of 3 non-SMCsubunits that define condensin II (Ono et al., 2003 [PubMed14532007]).
Background Reference 1:
Wood, J.L., et al. J. Biol. Chem. 283(43):29586-29592(2008)
Background Reference 2:
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Background Reference 3:
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Background Reference 4:
Smith, E.D., et al. Mol. Cell. Biol. 24(3):1168-1173(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-920

List Size:
400 ul

List Price:

$495.00

Datasheet
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