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Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.

NCAPH2 Antibody

CATALOG NUMBER: 27-036

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCAPH2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NCAPH2 antibody can be used for detection of NCAPH2 by ELISA at 1:62500. NCAPH2 antibody can be used for detection of NCAPH2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
721_B Cell Lysate
Predicted Molecular Weight:
68 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store NCAPH2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
NCAPH2
Additional Names:
NCAPH2, 384D8-2, 384D8_6, CAP-H2, MGC15858, MGC18000, MGC2455, MGC4133, MGC5305, MGC8640, hCAP-H2, CAPH2
Protein Accession Number:
NP_689512
Protein Gi Number:
148368978
Ncbi Gene Id Number:
29781
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4, but they contain different sets of non-SMC subunits. NCAPH2 is 1 of 3 non-SMC subunits that define condensin II.Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 (MIM 605576) and SMC4 (MIM 605575), but they contain different sets of non-SMC subunits. NCAPH2 is 1 of 3 non-SMC subunits that define condensin II (Ono et al., 2003 [PubMed 14532007]).
Background Reference 1:
Ono, T., (2003) Cell 115 (1), 109-121.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-036

List Size:
100 ul

List Price:

$519.00

Datasheet
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