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Western blot analysis of NCBP1 in HeLa cell lysate with NCBP1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of NCBP1 in HeLa cells with NCBP1 antibody at 2.5 μg/ml.
Western blot analysis of NCBP1 in HeLa cell lysate with NCBP1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NCBP1 Antibody

CATALOG NUMBER: 7395

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (81%)
Immunogen:
NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1.
The immunogen is located within amino acids 420 - 470 of NCBP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 87 kDa
Observed: 87 kDa
Purification:
NCBP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NCBP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NCBP1
Additional Names:
NCBP1 Antibody: NCBP, Sto1, CBP80, NCBP, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein
Protein Accession Number:
NP_002477
Protein Gi Number:
4505343
Ncbi Gene Id Number:
4686
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).
Background Reference 1:
Ohno M, Kataoka N, and Shimura Y. A nuclear cap binding protein from HeLa cells. Nuc. Acid Res. 1990; 18:6989-95.
Background Reference 2:
Maquat LE, Hwang J, Sato H, et al. CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter. Cold Spring Harbor Symp. Quant. Biol. 2010; 75:127-34.
Background Reference 3:
Bier K, York A, and Fodor E. Cellular cap-binding proteins associate with influenza virus mRNAs. J. Gen. Virol. 92:1627-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7395

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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