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Western blot analysis of extracts of various cell lines, using NCF1 antibody (14-032) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of Raji cells, using 3 ug NCF1 antibody (14-032). Western blot was performed from the immunoprecipitate using NCF1 antibody (14-032) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using NCF1 antibody (14-032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

NCF1 Antibody

CATALOG NUMBER: 14-032

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human NCF1 (NP_000256.4).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
THP-1
Positive Control 2:
Raji
Positive Control 3:
HL-60
Positive Control 4:
Mouse spleen
Positive Control 5:
Mouse lung
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 45kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NCF1
Additional Names:
Ncf1, neutrophil cytosolic factor 1, NOXO2, Ncf-1, p47, p47phox, 47 kDa neutrophil oxidase factor, NADPH oxidase subunit (47 kD), NADPH oxidase subunit (47kDa), NCF-47K, OTTMUSP00000022112, neutrophil NADPH oxidase factor 1, neutrophil cytosol facto
Ncbi Gene Id Number:
653361
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-032

List Size:
100 uL

List Price:

$436.00

Datasheet
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