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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis of NCK1 using rabbit polyclonal NCK1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene.
NCK1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in K562 cell line lysates (35ug/lane).

NCK1 Antibody

CATALOG NUMBER: 57-226

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human NCK1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCK1
Additional Names:
Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alpha, NCK1, NCK
Protein Accession Number:
P16333
Protein Gi Number:
127962
Ncbi Gene Id Number:
4690
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one of the signalingand transforming proteins containing Src homology 2 and 3 (SH2 andSH3) domains. It is located in the cytoplasm and is an adaptorprotein involved in transducing signals from receptor tyrosinekinases to downstream signal recipients such as RAS. Alternativelyspliced transcript variants encoding different isoforms have beenfound.
Background Reference 1:
Barda-Saad, M., et al. EMBO J. 29(14):2315-2328(2010)
Background Reference 2:
Gehmlich, K., et al. Eur. J. Cell Biol. 89(5):351-364(2010)
Background Reference 3:
Preisinger, C., et al. Cell. Signal. 22(5):848-856(2010)
Background Reference 4:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-226

List Size:
400 ul

List Price:

$495.00

Datasheet
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