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Western blot analysis in Ramos cell line lysates (35ug/lane).
NCK2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Ramos cell line lysates (35ug/lane).

NCK2 Antibody

CATALOG NUMBER: 57-480

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This NCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human NCK2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NCK2
Additional Names:
Cytoplasmic protein NCK2, Growth factor receptor-bound protein 4, NCK adaptor protein 2, Nck-2, SH2/SH3 adaptor protein NCK-beta, NCK2, GRB4
Protein Accession Number:
O43639
Ncbi Gene Id Number:
8440
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the NCK family of adaptorproteins. The protein contains three SH3 domains and one SH2domain. The protein has no known catalytic function but has beenshown to bind and recruit various proteins involved in theregulation of receptor protein tyrosine kinases. It is throughthese regulatory activities that this protein is believed to beinvolved in cytoskeletal reorganization. Alternate transcriptionalsplice variants, encoding different isoforms, have beencharacterized.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Guan, S., et al. J. Invest. Dermatol. 129(8):1909-1920(2009)
Background Reference 3:
Liu, J., et al. PLoS ONE(11), E7805 (2009) :
Background Reference 4:
Akiyama, M., et al. BrOphthalmol 92(9):1293-1296(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-480

List Size:
400 ul

List Price:

$495.00

Datasheet
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